Human Pendrin (SLC26A4) ELISA Kit

SKU: AE18853HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: O43511

Abbreviation: SLC26A4

Alternative Names: DFNB4; EVA; PDS; TDH2B; pendrin

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.128 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate SLC26A4 in samples. An antibody specific for SLC26A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC26A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC26A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC26A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Pendrin is an ion exchanger found in the cortical collecting duct.Pendrin is found at the luminal membrane of follicular cells in the thyroid gland. It transports iodine from the cytoplasm to the follicle lumen. Its activity is necessary for production of thyroid hormone.Pendrin is also found in the cells of the inner ear, where its function is not well understood. Mutations in this gene are associated with Pendred syndrome, the most common form of syndromic deafness, an autosomal-recessive disease. Pendred syndrome is also characterized by hypothyroidism. SLC26A4 is highly homologous to the SLC26A3 gene; they have similar genomic structures and this gene is located 3' of the SLC26A3 gene. The encoded protein has homology to sulfate transporters.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).