Human NEB(Noradrenaline Bitartas) ELISA Kit

SKU: ELK0075

Size:96T

Reactivity:Human

Alternative Names:(R)-(−)-norepinephrine; (R)-4-(2-amino-1-hydroxyethyl)-1,2-benzenediol; (R)-noradrenaline

Assay Type:Competitive Inhibition

Sensitivity:2.1 ng/mL

Standard:400 ng/mL

Detection range:6.25-400 ng/mL

Sample type:Serum, plasma, other biological fluids

Assay length:3.5h

Research Area:Signal Transduction

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NEB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: