Human Mixed Lineage Kinase Domain-like Protein, MLKL ELISA Kit

SKU: E1875Hu

Size:96 Tests-1 Kit

Background:Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 24316671). Does not have protein kinase activity (PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 24316671). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed: 29883610).

Research_area:Others

Applications:ELISA

Target_protein:MLKL

Reactivity:Human

Assay type:Sandwich

Sensitivity:11.18ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Mixed Lineage Kinase Domain-Like Protein

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q8NB16

MASS(Da):54,479

GeneID:197259

Synonyms:hMLKL;Mixed lineage kinase domain-like protein;MLKL

Gene names:MLKL

Standard solution:4800ng/L