Human Mesencephalic Astrocyte-derived Neurotrophic Factor, MANF ELISA Kit

SKU: E3041Hu

Size:96 Tests-1 Kit

Background:Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057).

Research_area:Neuroscience

Applications:ELISA

Target_protein:MANF

Reactivity:Human

Assay type:Sandwich

Sensitivity:5.39ng/L

Detection range:10-3800ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Mesencephalic astrocyte-derived neurotrophic factor

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P55145

MASS(Da):20,700

GeneID:7873

Synonyms:Arginine-rich protein;ARMET;ARP;MANF;Mesencephalic astrocyte-derived neurotrophic factor;Protein ARMET

Gene names:MANF

Standard solution:4000ng/L