Human Leukocyte antigen G (HLA-G) ELISA Kit

SKU: AE39914HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P17693

Abbreviation: HLA-G

Alternative Names: DADB-15K14.8; MHC-G; HLA class I molecule|HLA-G histocompatibility antigen; class I; G|MHC class I antigen|b2 microglobulin

Application: ELISA

Range: 1.56-100 ng/mL

Sensitivity: 0.8 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate HLA-G in samples. An antibody specific for HLA-G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHLA-G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HLA-G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HLA-G bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).