Human KiSS-1 receptor (KISS1R) ELISA Kit

SKU: AE36670HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q969F8

Abbreviation: KISS1R

Alternative Names: AXOR12; GPR54; HOT7T175; G protein-coupled receptor 54|metastin receptor

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.115 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate KISS1R in samples. An antibody specific for KISS1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKISS1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KISS1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KISS1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Kisspeptin is encoded by the metastasis suppressor gene KISS1, which is expressed in a variety of endocrine and gonadal tissues. Activation of the kisspeptin receptor is linked to the phospholipase C and inositol trisphosphate second messenger cascades inside the cell. Kisspeptin is involved in the regulation of endocrine function and the onset of puberty, with activation of the kisspeptin receptor triggering release of gonadotropin-releasing hormone (GnRH), and release of kisspeptin itself being inhibited by oestradiol but enhanced by GnRH. Reductions in kisspeptin levels with age may conversely be one of the reasons behind age-related declines in levels of other endocrine hormones such as luteinizing hormone.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).