Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit

SKU: ELK8534

Size:96T

Reactivity:Human

Alternative Names:C-RP; PTX1; Pentraxin-Related; Pentraxin 1; hs-CRP

Assay Type:Sandwich

Sensitivity:0.5 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

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