Human Granzymes B, GZMS-B ELISA Kit

SKU: E0899Hu

Size:96 Tests-1 Kit

Background:Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed: 3262682, PubMed: 3263427, PubMed: 1985927). It cleaves after Asp (PubMed: 8258716, PubMed: 1985927). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed: 32188940, PubMed: 31953257). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed: 9852092).

Research_area:Cell Biology

Applications:ELISA

Target_protein:GZMB

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.22ng/L

Detection range:0.5-300ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Granzymes B

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P10144

MASS(Da):27,716

GeneID:3002

Synonyms:C11;Cathepsin G-like 1;CTLA-1;CTSGL1;Cytotoxic T-lymphocyte proteinase 2;Fragmentin-2;Granzyme B;Granzyme-2;GZMB;HLP;Human lymphocyte protein;Lymphocyte protease;SECT;T-cell serine protease 1-3E

Gene names:GZMB

Standard solution:320ng/L