Human Glyceraldehyde-3-phosphate Dehydrogenase, GAPDH ELISA Kit

SKU: E5348Hu

Size:96 Tests-1 Kit

Background:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

Research_area:Cancer

Applications:ELISA

Target_protein:GAPDH

Reactivity:Human

Assay type:Sandwich

Sensitivity:9.61ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Glyceraldehyde-3-phosphate dehydrogenase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P04406

MASS(Da):36,053

GeneID:2597

Synonyms:G3PD;GAPD;GAPDH;Glyceraldehyde-3-phosphate dehydrogenase;HEL-S-162eP;Peptidyl-cysteine S-nitrosylase GAPDH

Gene names:GAPDH

Standard solution:4800ng/L