Human F13B(Coagulation Factor XIII B Polypeptide) ELISA Kit

SKU: ELK10620

Size:96T

Reactivity:Human

Alternative Names:F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain

Assay Type:Competitive Inhibition

Sensitivity:1.24 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:plasma

Assay length:2h

Research Area:Signal transduction;Metabolic pathway;Hematology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P05160