Human Egl Nine Homolog 1, EGLN1 ELISA Kit

SKU: E2500Hu

Size:96 Tests-1 Kit

Background:Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.

Research_area:Cardiovascular

Applications:ELISA

Target_protein:EGLN1

Reactivity:Human

Assay type:Sandwich

Sensitivity:10.14ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Egl Nine Homolog 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9GZT9

MASS(Da):46,021

GeneID:54583

Synonyms:C1orf12;ECYT3;Egl nine homolog 1;EGLN1;HALAH;HIF-PH2;HIF-prolyl hydroxylase 2;HPH2;HPH-2;Hypoxia-inducible factor prolyl hydroxylase 2;PHD2;Prolyl hydroxylase domain-containing protein 2;SM20;SM-20;ZMYND6

Gene names:EGLN1

Standard solution:4800ng/L