Human Dickkopf-related protein 2 (DKK2) ELISA Kit

SKU: AE47186HU

Species Reactivity:Human (Homo sapiens)

UniProt:Q9UBU2

Abbreviation:DKK2

Alternative Names:DKK-2; dickkopf 2|dickkopf homolog 2|dickkopf related protein-2

Application:ELISA

Range:78.1-5000 pg/mL

Sensitivity:31 pg/mL

Intra-AssayCV:?4.8%

Inter-AssayCV:?8.2%

Recovery:1

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate DKK2 in samples. An antibody specific for DKK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDKK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:Dickkopf-related protein 2 is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).