Human Deoxyribonuclease Gamma, DNASE1L3 ELISA Kit

SKU: E5176Hu

Size:96 Tests-1 Kit

Background:Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed: 9070308, PubMed: 9714828, PubMed: 14646506, PubMed: 10807908, PubMed: 27293190). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed: 23229555, PubMed: 24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed: 27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:DNASE1L3

Reactivity:Human

Assay type:Sandwich

Sensitivity:7.55ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Deoxyribonuclease Gamma

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q13609

MASS(Da):35,504

GeneID:1776

Synonyms:Deoxyribonuclease gamma;Deoxyribonuclease I-like 3;DNASE 1L3;DNase gamma;DNase I homolog protein DHP2;DNase I-like 3;DNASE1L3;Liver and spleen DNase;LSD;LS-DNAse

Gene names:DNASE1L3

Standard solution:3200ng/L