Human CST7(Cystatin-F) Microsample ELISA Kit

SKU: ELK10031MS

Size:96T

Reactivity:Human

Alternative Names:CST7;Cystatin-F;Cystatin-7;Cystatin-like metastasis-associated protein ;CMAP;Leukocystatin

Assay Type:Sandwich

Sensitivity:0.152 ng/mL

Standard:40 ng/mL

Detection range:0.625-40 ng/mL

Sample type:Serum, plasma,cell culture supernates

Assay length:3.5h

Research Area:Cell Biology

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CST7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CST7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CST7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CST7 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O76096