Human Cst Complex Subunit Stn1, OBFC1 ELISA Kit

SKU: E5128Hu

Size:96 Tests-1 Kit

Background:Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22964711, PubMed:22763445). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins (PubMed:25483097). Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres (PubMed:22863775, PubMed:22964711). Promotes general replication start following replication-fork stalling implicating new origin firing (PubMed:22863775). May be in involved in C-strand fill-in during late S/G2 phase independent of its role in telomere duplex replication (PubMed:23142664). Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites.

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:OBFC1

Reactivity:Human

Assay type:Sandwich

Sensitivity:5.62ng/L

Detection range:10-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:CST complex subunit STN1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9H668

MASS(Da):42,119

GeneID:79991

Synonyms:AAF44;AAF-44;bA541N10.2;CST complex subunit STN1;OBFC1;Oligonucleotide/oligosaccharide-binding fold-containing protein 1;RPA-32;Suppressor of cdc thirteen homolog

Gene names:STN1

Standard solution:2400ng/L