Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®

SKU: EK1422

Size: 96 wells/kit, with removable strips.

Storage: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Form:

Reactivity: Human

Applications: ELISA

Application Details:

Gene Name: NT5E

Specificity: Natural and recombinant human CD73

Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Immunogen: Expression system for standard: CHO; Immunogen sequence: W27-K547

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Uniprot ID: Q6NZX3

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Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

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Gene ID: 4907

Protein Name: 5'-nucleotidase

Gene Full Name: 5'-nucleotidase ecto

Synonyms: 5 NT; 5 nucleotidase; 5' nucleotidase; ecto (CD73); CD 73; CD73; E5NT; Ecto 5 nucleotidase; ecto-5'-NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NT5E/CD73; NTE