Human Arachidonate 12-lipoxygenase, 12S-type,ALOX12;LOG12 ELISA KIT

SKU: E4980Hu

Size:96 Tests-1 Kit

Background:Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:1851637, PubMed:8319693, PubMed:8500694, PubMed:18311922, PubMed:32404334). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA (PubMed:23504711). Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:9751607, PubMed:16638750, PubMed:22237009). Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768). {ECO:0000250|UniProtKB:P39655, ECO:0000269|PubMed:16638750, ECO:0000269|PubMed:17493578, ECO:0000269|PubMed:18311922, ECO:0000269|PubMed:1851637, ECO:0000269|PubMed:22237009, ECO:0000269|PubMed:22984144, ECO:0000269|PubMed:23504711, ECO:0000269|PubMed:23578768, ECO:0000269|PubMed:24282679, ECO:0000269|PubMed:32404334, ECO:0000269|PubMed:8250832, ECO:0000269|PubMed:8319693, ECO:0000269|PubMed:8500694, ECO:0000269|PubMed:9751607}.

Research_area:

Applications:ELISA

Target_protein:ALOX12

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.23ng/ml

Detection range:0.5-150ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Arachidonate 12-lipoxygenase, 12S-type

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P18054

MASS(Da):75694

GeneID:239

Synonyms:ALOX12 12LO LOG12

Gene names:ALOX12

Standard solution:160ng/ml