HiScript IV RT SuperMix for qPCR (+gDNA wiper)

SKU: R423-01

Size: 100 rxns 

Product Description  

HiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 × gDNA wiper Mix can completely remove the genomic contaimination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4 × HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH₂O to start the reaction.

 Features  

• Superior reverse transcription efficiency: Achieve qPCR detection results with smaller C_T values. 

• Great gDNA elimination efficiency: Thoroughly remove 500 ng of gDNA to ensure the accuracy of qPCR detection. 

• Excellent template compatibility: Efficient reverse transcription of low-input, low-expression or degraded RNA templates.

 Components  

 Storage  

 Store at -30 ~ -15°C and transport at ≤0°C.

 Performance  

Faster Reaction

Superior RT Efficiency

100 ng RNA from animals, plants and microbes were reverse transcribed by Vazyme #R423 and similar RT products from Supplier A,  Supplier B and Supplier C, following their respective manuals. The RT efficiency of these reagents was evaluated by qPCR amplification of obtained cDNA. It can be seen from the figure, △CT between Vazyme #R423 and Supplier C is within 0.5, indicating a  similar efficiency of them; △C_T between Vazyme #R423 and Suppler A/Supplier B are more than 0.5, indicating a better efficiency of Vazyme #R423.

Great gDNA Elimination Efficiency

500 ng gDNA from 293T cells were eliminated by Vazyme #R423 and similar RT products from Supplier A, Supplier B and  Supplier C. The digested gDNA were amplified by qPCR to evaluate the elimination efficiency of these products. The results  show that the gDNA elimination efficiency of Vazyme #R423 is consistent with that of Supplier A and Supplier B, and significantly  better than that of Supplier C.

Excellent Template Compatibility

100 ng human FFPE samples and degraded 293T cells (Rin=1) were reverse transcribed by Vazyme #R423 and similar RT  products from Supplier A, Supplier B and Supplier C. The template compatibility of these reagents was evaluated by qPCR amplification of obtained cDNA. The results show that △CT between Vazyme #R423 and Suppliers are more than 0.5 in most genes, indicating a better template compatibility of Vazyme #R423.