HiPure SF Plant DNA Kit

HiPure SF Plant DNA Kit

D316402
Regular price
$127.00 USD
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$127.00 USD
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Introduction

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from100 mg simple plant
Applications PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Economic plant samples
Sample amount

Fresh / frozen plant samples: ≤100m

gDried plant / seed samples: ≤20mg

Elution volume ≥30μl
Time per run 30-50 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


Principle

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

  • Safe - require no phenol or chloroform extraction
  • Fast - several samples can be extracted in 30 minutes by silica technology
  • High purity - high quality DNA, completely remove inhibitors
  • High yield - silica technology can achieve the highest yield


Kit Contents

Contents D316402 D316403
Purification Times 50 Preps 250 Preps
RNase A
10 mg
50 mg
Protease Dissolve Buffer
1.8 ml
5 ml
Buffer SPL 30 ml 150 ml
Buffer PS 10 ml 50 ml
Buffer GW1
22 ml
110 ml
Buffer GW2* 12 ml 2 x 50 ml
Buffer AE
15 ml
60 ml
HiPure gDNA Mini Columns
50 2 x 125
2 ml Collection Tubes
50 2 x 125


Storage and Stability

RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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