HDL CLIA kit (Human)

SKU: SCB006Hu

Size: 96Tests

# of Times Cited in literature: -

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: HDL

Target Full Name: High Density Lipoprotein

Alternative Names: -

Target Species: Human

Uniprot: P02647

Gene ID: 335

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human HDL / High Density Lipoprotein

Method: Chemiluminescence

Detection principle: Double-antibody Sandwich

Detection range: 0.206-150ng/mL

Sensitivity: 0.093ng/mL

Assay Time: 2h, 40min

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Density Lipoprotein (HDL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Density Lipoprotein (HDL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of High Density Lipoprotein (HDL). No significant cross-reactivity or interference between High Density Lipoprotein (HDL) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Test principle: The microplate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein (HDL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to High Density Lipoprotein (HDL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the High Density Lipoprotein (HDL) level in the sample or standard.;

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;

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