GB-AMP™ PaCeR™ HP™ DNA polymerase was derived from Pfu DNA Polymerase, after several iterative rounds of protein engineering, the enzyme boasts the on-board unique extension factor, specificity-promoting factors and plateau-inhibiting factor. It is a Fast Pfu-derived polymerase.
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- Super Fidelity: 53-fold higher than Taq DNA Polymerase and 6-fold better than Pfu DNA polymerase
- Long Range capability: amplify fragments as long as 40 kbλ DNA, 20 kb genomic DNA and 10 kb cDNA
- Difficult PCR suitability: Amplify targets with as high as 85% GC
- Direct-PCR: resists inhibitors in crude lysates of bacteria, fungi, whole blood, cultured cells, plant tissues, etc.
- Superior Hot-Start enzyme, employing two MAb as molecular thermal switches of polymerase and proof-reading activities.
- Fast PCR: rate of synthesis more than 2x that of Taq DNA polymerase
In an internal comparison studies, GB-AMP™ PaCeR™ HP™ was compared in the following aspects with the leading all-round enzymes such as Phusion (Thermo) and eight other leading DNA polymerases for PCR:
- Success rate in amplifying human, mouse, rice, wheat, lambda and plasmid DNA, ranging in size from 305 to 14768 bp
- Sensitivity, i.e., ability to detect low copy templates,
- Speed of amplification with extension time as short as 1 s (for 450 bp) and 5 s (1135 bp)
- Amplification of High GC template with GC as high as 85%
GB-AMP™ PaCeR™ HP™ was clearly the best all-round enzyme for robustness, sensitivity, speed, high GC amplification and Direct PCR!
A tip on the use: The annealing temperature should be chosen based on the Tm values of the primers, in general, 55-65°C. See the user manual for details.
Looking for a similar DNA Polymerase with higher fidelity and amplification speed? Check out our 2× PaCeR (Phanta) Flash Master Mix (P510).
The enzyme generates blunt-ended products and thus products can be cloned by our Blunt End Cloning Kits.