FineTest®Violet 450 Anti-Mouse CD146 Antibody(ME-9F1)

SKU: FV450-30197

Size: 50T

Target: Mouse CD146

Form: liquid

Conjugation: FineTest®Violet 450

Conjugation Information: FineTest® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).

Purification: 0

Purity: 0

Host: Rat

Clonality: Monoclonal

Clone ID: ME-9F1

Isotype: IgG2a, κ

Storage: PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.

Immunogen: 0

Synonyms: S-Endo 1 antigen|MUC18|MCAM|Mel-CAM|A32 antigen

Observed MW:

Uniprot ID: Q8R2Y2

Reactivity: Mouse

Tested Application: FC

Recommended dilution: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

image5:

Gene ID: 84004

Research Area:

Catagory: Flow cytometry Antibodies