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Highlights |
• Mutation sites on both primers to improve mutation efficiency. • Partially overlapping primers for exponential DNA amplification. • Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification. • Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency. |
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Storage |
Competent Cell at -70°C for six months; others at -20°C for two years |
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Kit Content |
|
Component |
FM111-01 (10 rxns) |
FM111-02 (20 rxns) |
|
2×TransStart® FastPfu PCR SuperMix |
250 µl |
500 µl |
|
DMT Enzyme (10 units/μl) |
10 µl |
20 µl |
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DMT Chemically Competent Cell |
10×50 µl |
20×50 µl |
|
ddH2O |
1 ml |
1 ml |
|
SControl Plasmid (5 ng/µl) |
10 µl |
20 µl |
|
SControl Primers (10 µM ) |
10 µl |
20 µl |
Papers citing this product:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history. | Nature genetics | 29.648 | Xuming Zhou, et al. | 2014 Dec | The original link |
| Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs | Cell Research | 14.812 | Heqiao Zhang, et al. Ministry of Education Key Lab | 2016 jan | The original link |
| Maternal xNorrin, a canonical Wnt signaling agonist and TGF-β antagonist, controls early neuroectoderm specification in Xenopus | PloS Biology | 12.69 | Xu S, et al. State Key Laboratory of Molecular Dev | 2012 | The original link |
| PGE2 Promotes Hepatic Biliary Cholesterol Excretion by EP3-mediated HNF4α/CYP7A1 Pathway in Mice | hepatology | 11.711 | Shuai Yan,et al. | 2016Dec | The original link |
| Plastid-nucleus communication involves calcium-modulated MAPK signalling | Nature communications | 11.329 | Hailong Guo, et al. Photosynthesis Research Center | 2016 jun | The original link |