EasyStep Human OPG(Osteoprotegerin) ELISA Kit

SKU: ELK034ES

Size:96T

Reactivity:Human

Alternative Names:TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

Assay Type:Sandwich

Sensitivity:23.6 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:Serum

Assay length:1.5h

Research Area:Cytokine;Metabolic pathway;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPG, and the Human OPG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OPG. After TMB substrate solution is added, only those wells that contain Human OPG and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O00300