EasyPure® Blood Genomic DNA Kit (with RNase A)

SKU: EE121-01

Product Details

EasyPure^® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood. Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot.

Features

• Simple and fast, red cell lysis buffer is no longer needed.

• Complete removal of contaminants and inhibitors.

• DNA yield up to 40 μg.

• Column based purification, no organic extraction or ethanol precipitation.

• Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μl.

See manual for more details on EE121:

Storage

At room temperature (15-25°C) in a dry place for one year

Shipping

At room temperature

Product Composition

Component	EE121-01 (50 rxns)	EE121-02 (200 rxns)
Binding Buffer 3 (BB3)	25 ml	110 ml
Clean Buffer 3 (CB3)	6 ml	24 ml
Wash Buffer (WB3)	11 ml	2x22 ml
Elution Buffer (EB)	20 ml	80 ml
RNase A (20 mg/ml)	500 ul	2x1 ml
Proteinase K (20 mg.ml)	1 ml	4x1 ml
Genomic Spin Columns with Collection Tubes	50 each	200 each

Procedure

Component	EE121-01 (50 rxns)	EE121-02 (200 rxns)
Clean Buffer 3 (CB3)	24 ml	96 ml
Wash Buffer 3 (WB3)	44 ml	2x88 ml

References

1 Zhang D Y, Zhang X X, Li F D, et al. Whole-genome resequencing reveals molecular imprints of anthropogenic and natural selection in wild and domesticated sheep[J]. Zoological Research, 2022.(IF 6.97)

2 Zhang D, Zhang X, Li F, et al. Whole-genome resequencing identified candidate genes associated with the number of ribs in Hu sheep[J]. Genomics, 2021.(IF 6.20)