E.coli Poly (A) Polymerase

SKU: LA101-01

Product Details

This product is purified from E. coli after inducible expression of a recombinant vector containing Poly (A) Polymerase gene. It has a molecular weight of 50.2 kDa. The reaction of E. coli Poly (A) Polymerase is template-independent. It catalyzes the addition of AMP converted from ATP to the 3'end of the RNA, that is, adding a poly (A) tail to the RNA.

Highlights

Template independent addition of a poly (A) tail to the 3'end of the RNA.

Application

Add a poly(A) tail for reverse transcription into cDNA; labeling RNA with modified ATP or its analogues.

Storage

at -20°C for two years

Shipping

Dry ice (-70 ℃)

Product Contents

Component	LA101-01	LA101-02
E.coli Poly (A) Polymerase	100 units	500 units
10×E.coli Poly (A) Polymerase Reaction Buffer	80 μl	400 μl
10×DNA Loading Buffer	1 ml	1 ml