DNA Library Prep Kit for Oxford Nanopore Technologies

SKU: TS201G

VAHTS TGS DNA Library Prep Kit for Oxford Nanopore Technologies ONT

Size: 24 Rxns.

Keywords: DNA library construction; third generation library construction; TGS library construction; Nanopore sequencing; ONT sequencing

VAHTS TGS DNA Library Prep Kit for ONT is a ligation-based library construction kit suitable for Nanopore sequencing platform. Combined with the Barcode Adapter, it can perform single-sample or multi-sample analysis of 100 ng - 1.5 μg gDNA and amplicons. Build a database. This kit uses a directed evolution mutant enzyme with high catalytic activity and a carefully optimized buffer. It can directly perform Barcode Ligation without purification after end repair/damage repair/A-tail addition, and the experimental process is convenient. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction.

Features:

1) The operation process is simple: steps of end repair + damage repair + adding A tail are completed in one tube, no purification is required, and Barcode connection can be performed directly

2) The time required for library construction is shortened: the minimum time required for the entire process of database construction can be <2 hours, which saves ≥30 minutes compared with the conventional process.

3) Compatible with various species you need to work with: compatible with animals, plants, microorganisms and other species

4) Excellent data quality: high read rate of long fragments, high data output, and good data quality

Better Data output and Quality

Species	Metric	TS201	Supplier A
Human	Clean Total Reads	1,910,636	1,754,451
	Clean Total bases	37,568,318,787	30,769,214,717
	Clean Mean quality(Mean Q)	14.77	14.69
Plant	Clean Total Reads	3,398,731	3,494,821
	Clean Total bases	32,590,018,744	31,687,125,980
	Clean Mean quality(Mean Q)	12.86	12.77
Bacteria	Clean Total Reads	223,825	287,405
	Clean Total bases	1,629,142,727	1,791,295,356
	Clean Mean quality(Mean Q)	15.5	15.3

Better Longer Reads

Species	DNA Size	TS201	Supplier A
Human	5 - 50 kb	63.63%	60.95%
	50-100 kb	8.25%	6.57%
	100-200 kb	0.13%	0.11%
	Total	72.01%	67.63%
Plant	5 - 50 kb	39.63%	37.02%
	50-100 kb	2.22%	2.10%
	100-200 kb	0.02%	0.02%
	Total	41.87%	39.14%

Kit composition

Component	TS201-01 (24 rxns)
End Prep & Repair Buffer	36 µL
End Prep & Repair Master Enzyme Mix	36 µL
Barcode Rapid Ligase Mix	96 µ L
MP Adapter Fast Ligation Buffer	240 µL
MP Adapter Fast Ligase	120 µL