DHT ELISA kit (General species)

SKU: CEA443Ge

Size: 96Tests

# of Times Cited in literature: 9

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: DHT

Target Full Name: Dihydrotestosterone

Alternative Names: 5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species DHT / Dihydrotestosterone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 30.9-2,500pg/mL

Sensitivity: 10.7pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydrotestosterone (DHT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydrotestosterone (DHT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Dihydrotestosterone (DHT). No significant cross-reactivity or interference between Dihydrotestosterone (DHT) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dihydrotestosterone (DHT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dihydrotestosterone (DHT) and unlabeled Dihydrotestosterone (DHT) (Standards or samples) with the pre-coated antibody specific to Dihydrotestosterone (DHT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dihydrotestosterone (DHT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dihydrotestosterone (DHT) in the sample.

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

References Citing This Product: Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar Rats

Metabolic Changes and Hormonal Disturbances in Polycystic Ovarian Syndrome Rats and the Amelioration Effects of Metformin and/or Cinnamon Extraction

Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal model

Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2

Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα

Dihydrotestosterone Treatment Accelerates Autograft Reversal Sciatic Nerve Regeneration in Rats

Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome

Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression

Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells