GeneBio Systems

COL1 ELISA kit (Rat)

Name: COL1 ELISA kit (Rat)
Brand: GeneBio Systems
SKU: SEA571Ra
Price: 1187.00 USD
Availability: InStock

SKU:SEA571Ra

$1,187.00 USD

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Quantity

Size: 96Tests

# of Times Cited in literature: 20

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: COL1

Target Full Name: Collagen Type I

Alternative Names: -

Target Species: Rat

Uniprot: P02454 & P02466

Gene ID: 29393 & 84352

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat COL1 / Collagen Type I

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.625-40ng/mL

Sensitivity: 0.224ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I (COL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I (COL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type I (COL1). No significant cross-reactivity or interference between Collagen Type I (COL1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I (COL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I (COL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I (COL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I (COL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Metabolic pathway;Developmental science;Bone metabolism;

References Citing This Product: Urotensin II induces phenotypic differentiation, migration, and collagen synthesis of adventitial fibroblasts from rat aorta

Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans

In vitro tenocyte metabolism in aging and oestrogen deficiency

Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats

The unwounded skin remodeling in animal models of diabetes types 1 and 2.

Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet

KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload

NIM811 downregulates transforming growth factor

Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats

Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue

Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition