CFSE Cell Division Tracker Kit

SKU: E-CK-A345

Size:2000Assays

Storage:This product can be stored at -20°C for 12 months with shading light.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Proliferation and Cycle

Category ID_III:CFSE Cell Proliferation Assay Kits

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Kit Type:Cell Proliferation

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Detection Instrument:Flow Cytometer;Fluorescence Microscope

Tested Sample Types:Cell samples

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Detection Type:Fluorometric method

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Test Principle:CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps.

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