Cattle IL12A(Interleukin 12A) ELISA Kit

SKU: ELK5873

Size:96T

Reactivity:Cattle

Alternative Names:IL-12A;p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1

Assay Type:Sandwich

Sensitivity:3.2 pg/mL

Standard:500 pg/mL

Detection range:7.82-500 pg/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Cytokine;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL12A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL12A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL12A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL12A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P54349