Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit

SKU: ELK10693

Size:96T

Reactivity:Cattle

Alternative Names:BP4; HT29-IGFBP; IBP4

Assay Type:Competitive Inhibition

Sensitivity:0.56 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Apoptosis;Tumor immunity;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q05716