Caspase 9 Activity Assay Kit(Colorimetric Method)

SKU: E-CK-A389

Size:100Assays

Storage:This product can be stored at -20°C for 12 months with shading light.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Apoptosis

Category ID_III:Caspase Assay Kits

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Kit Type:Apoptosis/Caspase

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Result Type:Qualitative or quantitative

Detection Instrument:Spectrophotometer;Microplate reader

Tested Sample Types:Cell and tissue samples

Assay Time:4-6 hours

Detection Type:Colorimetric method

Background:

Test Principle:Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm.

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