Caspase 2 Activity Assay Kit(Colorimetric Method)

SKU: E-CK-A382

Size:100Assays

Storage:This product can be stored at -20°C for 12 months with shading light.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Apoptosis

Category ID_III:Caspase Assay Kits

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Kit Type:Apoptosis/Caspase

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Result Type:Qualitative or quantitative

Detection Instrument:Spectrophotometer;Microplate reader

Tested Sample Types:Cell and tissue samples

Assay Time:4-6 hours

Detection Type:Colorimetric method

Background:

Test Principle:Caspase 2, also known as Ich-1(ICE and CED-3 homolog 1) or Nedd-2, can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different forms. The mRNA product of full-length caspase 2 can be promote apoptosis, while the protein product of short mRNA can inhibit apoptosis. Caspase 2 can be activated by caspase 1, caspase 3 and granzyme B in vitro. This caspase 2 activity assay kit is based on caspase 2 that can catalyze the substrate Ac-VDQQD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 2 can be calculated by determining the absorbance at 405 nm.

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