CASP8 Monoclonal Antibody

SKU: E-AB-22107

Size: 200μL

Storage: Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping: The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date: 12 months

Category ID_II: Primary Antibodies

Category ID_III: Monoclonal Antibodies

Abbreviation: Caspase-8

Target Synonym: ALPS2B;Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein;Apoptotic cysteine protease;Apoptotic protease Mch-5;Apoptotic protease Mch5;CAP4;CASP-8;CASP8;CASP8;Caspase 8;Caspase 8 apoptosis related cysteine peptidase;Caspase-8 subunit p10;CED 3;FADD Like ICE;FADD-homologous ICE/CED-3-like protease;FADD-like ICE;FLICE;FLJ17672;ICE-like apoptotic protease 5;MACH alpha 1/2/3 protein;MACH;MACH beta 1/2/3/4 protein;MCH5;MGC78473;MORT1 associated ced 3 homolog;MORT1-associated CED-3 homolog;OTTHUMP00000163717;OTTHUMP00000163720;OTTHUMP00000163724;OTTHUMP00000163725;OTTHUMP00000165062;OTTHUMP00000165063;OTTHUMP00000165064;OTTHUMP00000206552;OTTHUMP00000206582

Research Areas: Cancer;Cell Biology;Metabolism

Conjugation: Unconjugated

Host: Mouse

Species reactivity: Human;Mouse;Rat

Application: WB;IHC-p;IF

Isotype: IgG

Clonality: Monoclonal

Clone NO.: Clone:2E3

UNIProt ID: Q14790

Accession:

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Concentration: 1 mg/mL

Immunogen: Recombinant Protein

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification method: Protein A purification

Dilution: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:100-1:300

Calculated MW:

ObservedMW: 43,57kDa