Bovine Cytochrome P450 17A1,CYP17A1 ELISA KIT

SKU: E0081Bo

Size:96Tests

Background:A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). {ECO:0000250|UniProtKB:P05093}.

Research_area:

Applications:ELISA

Target_protein:CYP17A1

Reactivity:Bovine

Assay type:Sandwich

Sensitivity:0.26ng/ml

Detection range:0.5-32ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Steroid 17-alpha-hydroxylase;17,20 lyase;Cytochrome P450 17A1

Species:Bovine

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P05185

MASS(Da):57244

GeneID:281739

Synonyms:CYP17A1 CYP17

Gene names:CYP17A1

Standard solution:64ng/ml