Bax ELISA kit (Human)

SKU: SEB343Hu

Size: 96Tests

# of Times Cited in literature: 12

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: Bax

Target Full Name: Bcl2 Associated X Protein

Alternative Names: BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

Target Species: Human

Uniprot: Q07812

Gene ID: 581

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human Bax / Bcl2 Associated X Protein

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 62.5-4,000pg/mL

Sensitivity: 28.2pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2 Associated X Protein (Bax). No significant cross-reactivity or interference between Bcl2 Associated X Protein (Bax) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein (Bax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated X Protein (Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein (Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein (Bax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Apoptosis;

References Citing This Product: The combined treatment with novel platinum(II) complex and anti-MUC1 increases apoptotic response in MDA-MB-231 breast cancer cells

Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms

Mechanism of anticancer action of novel berenil complex of platinum(II) combined with anti-MUC1 in MCF-7 breast cancer cells

Mechanism of anticancer action of novel berenil complex of platinum (II) combined with anti-MUC1 in MCF-7 breast cancer cells

Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress Disorder

Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells

Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent)