b-Enolase Antibody , Cat. # AF4099

SKU: AF4099

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:ENO3

GeneID:2027

Uniprot:P13929

Specificity:b-Enolase Antibody detects endogenous levels of total b-Enolase.

Immunogen:A synthesized peptide derived from human b-Enolase(ENO-3), corresponding to a region within C-terminal amino acids.

Storage:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reactivity:Human,Mouse

Prediction:Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Application:WB,IHC,IF/ICC,ELISA(peptide)

Dilution:WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2835357

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF4099-BP

Des# Blocking Peptide:b-Enolase Blocking peptide

Blocking Peptide Price:$549 CAD