Anti-XPB/ERCC3 Antibody Picoband®

SKU: A03103-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ERCC3

Specificity:

Background: XPB (xeroderma pigmentosum type B) is an ATP-dependent DNA helicase in humans that is a part of the TFIIH transcription factor complex. This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human XPB/ERCC3 recombinant protein (Position: K68-Q377).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Coin, F., Oksenych, V., Egly, J.-M. Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repair. Molec. Cell 26: 245-256, 2007. 2. Kim, T.-K., Ebright, R. H., Reinberg, D. Mechanism of ATP-dependent promoter melting by transcription factor IIH. Science 288: 1418-1421, 2000. 3. Le May, N., Dubaele, S., De Santis, L. P., Billecocq, A., Bouloy, M., Egly, J.-M.TFIIH transcription factor, a target for the Rift Valley hemorrhagic fever virus. Cell 116: 541-550, 2004.

Uniprot ID: P19447

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 89 kDa

Calculated Molecular Weight: 64406 MW

Gene ID: 2071

Protein Name: General transcription and DNA repair factor IIH helicase/translocase subunit XPB

Gene Full Name: ERCC excision repair 3, TFIIH core complex helicase subunit

Synonyms: BTF2; BTF2 p89; ERCC3; GTF2H; RAD25; TFIIH; TFIIH 89 kDa subunit; TFIIH p89; XPB; XPBC