Anti-Villin/VIL1 Antibody Picoband®

SKU: PB9457

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunofluorescence, 2μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: VIL1

Specificity: No cross reactivity with other proteins.

Background: Villin is known as VIL1. This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. In vertebrates, the villin proteins help to support the microfilaments of the microvilli of the brush border. It may play a role in cell plasticity through F-actin severing.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Villin, different from the related mouse sequence by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Friederich, E; Vancompernolle, K; Louvard, D; Vandekerckhove, J (1999). Villin function in the organization of the actin cytoskeleton. Correlation of in vivo effects to its biochemical activities in vitro. The Journal of Biological Chemistry274 (38): 26751–60. 2. Meng, J; Vardar, D; Wang, Y; Guo, HC; Head, JF; McKnight, CJ (2005). High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity. Biochemistry 44 (36): 11963–73.

Uniprot ID: P09327

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 93 kDa

Calculated Molecular Weight: 92695 MW

Gene ID: 7429

Protein Name: Villin-1

Gene Full Name: villin 1

Synonyms: VIL; VIL1; Villin; villin 1