Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband®

SKU: A07355-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,WB

Application Details: Western blot, 0.1-0.25μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunofluorescence, 5 μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: TNNI2

Specificity: No cross reactivity with other proteins.

Background: Troponin I, fast skeletal muscle is a protein that in humans is encoded by the TNNI2 gene. It is mapped to 11p15.5. This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human Troponin I fast skeletal muscle/TNNI2 recombinant protein (Position: D3-S182).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Barton, P. J. R., Townsend, P. J., Brand, N. J., Yacoub, M. H. Localization of the fast skeletal muscle troponin I gene (TNNI2) to 11p15.5: genes for troponin I and T are organized in pairs. Ann. Hum. Genet. 61: 519-523, 1997. 2. Drera, B., Zoppi, N., Barlati, S., Colombi, M. Recurrence of the p.R156X TNNI2 mutation in distal arthrogryposis type 2B. (Letter) Clin. Genet. 70: 532-534, 2006.

Uniprot ID: P48788

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 24 kDa

Calculated Molecular Weight: 108320 MW

Gene ID: 7136

Protein Name: Troponin I, fast skeletal muscle

Gene Full Name: troponin I2, fast skeletal type

Synonyms: AMCD2B; DA2B; FSSV; fsTnI; TNNI2; Troponin I fast skeletal muscle; Troponin I; fast-twitch isoform