Anti-TRMT5 Antibody Picoband®

SKU: A11359-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TRMT5

Specificity:

Background: tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.

Immunogen: E.coli-derived human TRMT5 recombinant protein (Position: R291-E501).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Argente-Escrig, H., Vilchez, J. J., Frasquet, M., Muelas, N., Azorin, I., Vilchez, R., Millet-Sancho, E., Pitarch, I., Tomas-Vila, M., Vazquez-Costa, J. F., Mas-Estelles, F., Marco-Marin, C., Espinos, C., Serrano-Lorenzo, P., Martin, M. A., Lupo, V., Sevilla, T. A novel TRMT5 mutation causes a complex inherited neuropathy syndrome: The role of nerve pathology in defining a demyelinating neuropathy. Neuropath. Appl. Neurobiol. 48: e12817, 2022. 2. Brule, H., Elliott, M., Redlak, M., Zehner, Z. E., Holmes, W. M. Isolation and characterization of the human tRNA-(N(1)G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein. Biochemistry 43: 9243-9255, 2004. 3. Haller, R. G., Lewis, S. F., Estabrook, R. W., DiMauro, S., Servidei, S., Foster, D. W. Exercise intolerance, lactic acidosis, and abnormal cardiopulmonary regulation in exercise associated with adult skeletal muscle cytochrome c oxidase deficiency. J. Clin. Invest. 84: 155-161, 1989.

Uniprot ID: Q32P41

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight:

Gene ID: 57570

Protein Name: tRNA

Gene Full Name: tRNA methyltransferase 5

Synonyms: KIAA1393; M1G methyltransferase; TRM5; TRMT5; tRNA [GM37] methyltransferase; tRNA methyltransferase 5