Anti-Transferrin Receptor/TFRC Antibody Picoband®

SKU: PB9233

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,IHC-F,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: TFRC

Specificity: No cross reactivity with other proteins.

Background: Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. It is mapped to 3q29. TFRC is a transmembrane glycoprotein composed of two disulfide-linked monomers joined by two disulfide bonds. Expression of human TFR1 in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses. TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. It is required for iron delivery from transferrin to cells.

Immunogen: E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Sutherland R, Delia D, Schneider C, Newman R, Kemshead J, Greaves M (July 1981). Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin 2. Speeckaert MM, Speeckaert R, Delanghe JR (December 2010). Biological and clinical aspects of soluble transferrin receptor. Critical Reviews in Clinical Laboratory Sciences 47 (5-6): 213–228. 3. Radoshitzky, S. R., Abraham, J., Spiropoulou, C. F., Kuhn, J. H., Nguyen, D., Li, W., Nagel, J., Schmidt, P. J., Nunberg, J. H., Andrews, N. C., Farzan, M., Choe, H. Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses. Nature 446: 92-96, 2007.

Uniprot ID: P02786

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight: 84871 MW

Gene ID: 7037

Protein Name: Transferrin receptor protein 1

Gene Full Name: transferrin receptor

Synonyms: CD71; p90; sTfR; T9; TFR; TFR1; TFRC; TR; Transferrin receptor protein 1; TRFR