Anti-Transcription factor E2F2 E2F2 Antibody Picoband®

SKU: PA1810

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: E2F2

Specificity: No cross reactivity with other proteins.

Background: E2F2 (E2F transcription factor 2) also called E2F-2, is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F2 gene is mapped to 1p36 by fluorescence in situ hybridization. Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C. The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4. In contrast, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2, identical to the related mouse sequence.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Funke-Kaiser, H., Reichenberger, F., Kopke, K., Herrmann, S.-M., Pfeifer, J., Orzechowski, H.-D., Zidek, W., Paul, M., Brand, E. Differential binding of transcription factor E2F-2 to the endothelin-converting enzyme-1b promoter affects blood pressure regulation. Hum. Molec. Genet. 12: 423-433, 2003. 3. Lees, J. A., Saito, M., Vidal, M., Valentine, M., Look, T., Harlow, E., Dyson, N., Helin, K. The retinoblastoma protein binds to a family of E2F transcription factors. Molec. Cell. Biol. 13: 7813-7825, 1993.

Uniprot ID: Q14209

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 48 kDa

Calculated Molecular Weight: 47506 MW

Gene ID: 1870

Protein Name: Transcription factor E2F2

Gene Full Name: E2F transcription factor 2

Synonyms: E2F transcription factor 2; E2F-2; E2F2