Anti-TP53 Antibody Picoband®

SKU: A00001-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml/ml, Human

Gene Name: TP53

Specificity:

Background: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.

Immunogen: E.coli-derived human TP53 recombinant protein (Position: M1-D186).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aaltonen, L.-M., Chen, R. W., Roth, S., Makitie, A. A., Rihkanen, H., Vaheri, A., Aaltonen, L. A. Role of TP53 P72R polymorphism in human papillomavirus associated premalignant laryngeal neoplasm. J. Med. Genet. 38: 327 only, 2001. 2. Aas, T., Borresen, A.-L., Geisler, S., Smith-Sorenson, B., Johnsen, H., Varhaug, J. E., Akslen, L. A., Lonning, P. E. Specific P53 mutations are associated with de novo resistance to doxorubicin in breast cancer patients. Nature Med. 2: 811-814, 1996. 3. Abida, W. M., Nikolaev, A., Zhao, W., Zhang, W., Gu, W. FBXO11 promotes the neddylation of p53 and inhibits its transcriptional activity. J. Biol. Chem. 282: 1797-1804, 2007.

Uniprot ID: P04637

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 53 kDa

Calculated Molecular Weight:

Gene ID: 7157

Protein Name: Cellular tumor antigen p53

Gene Full Name: tumor protein p53

Synonyms: Antigen NY CO 13; Cellular tumor antigen p53; LFS1; P53; Phosphoprotein p53; TP53; TRP53; tumor protein p53; Tumor suppressor p53