Anti-SPON2 Antibody Picoband®

SKU: A07465-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPON2

Specificity:

Background: Spondin 2, also known as mindin, is a protein that in humans is encoded by the SPON2 gene. SPON2 (spondin 2, mindin) is an extracellular matrix protein belonging to the mindin/F-spondin family. The protein has two amino terminal follistatin-like (FS) domains and a carboxy terminal thrombospondin type 1 repeat (TSR) domain. The FS domains mediate interactions between SPON2 and integrins, whereas the TSR domain contributes to the role of SPON2 as a pathogen pattern recognition molecule. In that capacity, SPON2 plays an important role in initiation of the innate immune response. It functions as a pattern recognition molecule within the extracellular matrix, where it binds to microbial pathogens and presents them to immune cells. Interactions between SPON2 and integrins are also critical for inflammatory cell recruitment and T cell priming. In addition to its role in innate immunity, SPON2 has also been shown to play a role in hippocampal neuronal outgrowth, cancer metastasis, and diabetic nephropathy.

Immunogen: E.coli-derived human SPON2 recombinant protein (Position: Q53-V331).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. He, Y.-W., Li, H., Zhang, J., Hsu, C.-L., Lin, E., Zhang, N., Guo, J., Forbush, K. A., Bevan, M. J. The extracellular matrix protein mindin is a pattern-recognition molecule for microbial pathogens. Nature Immun. 5: 88-97, 2004. 2. Manda, R., Kohno, T., Matsuno, Y., Takenoshita, S., Kuwano, H., Yokota, J. Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display. Genomics 61: 5-14, 1999.

Uniprot ID: Q9BUD6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight: 51200 MW

Gene ID: 10417

Protein Name: Spondin-2

Gene Full Name: spondin 2

Synonyms: DIL 1; DIL1; M spondin; Mindin; SPON2; Spondin 2