Anti-SPO11 Antibody Picoband®

SKU: A04719-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: SPO11

Specificity:

Background: Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described.

Immunogen: E.coli-derived human SPO11 recombinant protein (Position: R40-D326). Human SPO11 shares 83.3% amino acid (aa) sequence identity with mouse SPO11.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Baudat, F., Manova, K., Yuen, J. P., Jasin, M., Keeney, S. Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11. Molec. Cell 6: 989-998, 2000. 2. Garcia, V., Phelps, S. E. L., Gray, S., Neale, M. J. Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1. Nature 479: 241-244, 2011. 3. Guillon, H., Baudat, F., Grey, C., Liskay, R. M., de Massy, B. Crossover and noncrossover pathways in mouse meiosis. Molec. Cell 20: 563-573, 2005.

Uniprot ID: Q9Y5K1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 45 kDa

Gene ID: 23626

Protein Name: Meiotic recombination protein SPO11

Gene Full Name: SPO11 initiator of meiotic double strand breaks

Synonyms: Cancer/testis antigen 35, CT35, SPO11