Anti-SPAG16 Antibody Picoband®

SKU: A09080-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPAG16

Specificity:

Background: Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively

Immunogen: E.coli-derived human SPAG16 recombinant protein (Position: D28-F619). Human SPAG16 shares 75.1% amino acid (aa) sequence identity with mouse SPAG16.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Pennarun, G., Bridoux, A.-M., Escudier, E., Dastot-Le Moal, F., Cacheux, V., Amselem, S., Duriez, B. Isolation and expression of the human hPF20 gene orthologous to Chlamydomonas pf20: evaluation as a candidate for axonemal defects of respiratory cilia and sperm flagella. Am. J. Resp. Cell Molec. Biol. 26: 362-370, 2002. 2. Wilson, C. W., Nguyen, C. T., Chen, M.-H., Yang, J.-H., Gacayan, R., Huang, J., Chen, J.-N., Chuang, P.-T. Fused has evolved divergent roles in vertebrate hedgehog signalling and motile ciliogenesis. Nature 459: 98-102, 2009. 3. Zhang, Z., Kostetskii, I., Moss, S. B., Jones, B. H., Ho, C., Wang, H., Kishida, T., Gerton, G. L., Radice, G. L., Strauss, J. F., III. Haploinsufficiency for the murine orthologue of Chlamydomonas PF20 disrupts spermatogenesis. Proc. Nat. Acad. Sci. 101: 12946-12951, 2004.

Uniprot ID: Q8N0X2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 71 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 79582

Protein Name: Sperm-associated antigen 16 protein

Gene Full Name: sperm associated antigen 16

Synonyms: PF20; Pf20 protein homolog; SPAG16; sperm associated antigen 16; WDR29