Anti-SnoN/SNO/SKIL Antibody Picoband®

SKU: A04131-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SKIL

Specificity:

Background: Ski-like protein is a protein that in humans is encoded by the SKIL gene. The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene.

Immunogen: E.coli-derived human SnoN/SNO/SKIL recombinant protein (Position: M1-V504).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 5/27/2014. 2. Nomura, N., Sasamoto, S., Ishii, S., Date, T., Matsui, M., Ishizaki, R. Isolation of human cDNA clones of SKI and the SKI-related gene, SNO. Nucleic Acids Res. 17: 5489-5500, 1989. 3. Pearson-White, S., Crittenden, R. Proto-oncogene Sno expression, alternative isoforms and immediate early serum response. Nucleic Acids Res. 25: 2930-2937, 1997.

Uniprot ID: P12757

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 77 kDa

Calculated Molecular Weight: 211344 MW

Gene ID: 6498

Protein Name: Ski-like protein

Gene Full Name: SKI like proto-oncogene

Synonyms: SKI like oncogene; Ski like protein; Ski related oncogene; Ski related protein; SKIL; SNO; SnoA; SnoI; SnoN