Anti-SMTNL2 Antibody Picoband®

SKU: A18269-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SMTNL2

Specificity:

Background: The cytoskeletal protein smoothelin is highly conserved among vertebrates and is expressed exclusively by contractile smooth muscle cells where it localizes to the filament network. Smoothelin associates with Actin stress fibers but does not interact with Desmin. SMTNL2 (smoothelin-like 2), also known as FLJ42461, MGC131847 or MGC138382, is a 461 amino acid protein belonging to the smoothelin family. Containing a single CH (calponin-homology) domain, SMTNL2 is considered an Actin binding protein. SMTNL2 exists as two isoforms produced by alternative splicing events and is encoded by a gene mapping to human chromosome 17. Human chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is ly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

Immunogen: E.coli-derived human SMTNL2 recombinant protein (Position: M1-K440). Human SMTNL2 shares 83.2% amino acid (aa) sequence identity with mouse SMTNL2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gordon, E. A., Whisenant, T. C., Zeller, M., Kaake, R. M., Gordon, W. M., Krotee, P., ... & Bardwell, L. (2013). Combining docking site and phosphosite predictions to find new substrates: identification of smoothelin-like-2 (SMTNL2) as a c-Jun N-terminal kinase (JNK) substrate. Cellular signalling, 25(12), 2518-2529. 2. Saxena, R., Plenge, R. M., Bjonnes, A. C., Dashti, H. S., Okada, Y., Gad El Haq, W., ... & Arayssi, T. (2017). A multinational Arab genome‐wide association study identifies new genetic associations for rheumatoid arthritis. Arthritis & Rheumatology, 69(5), 976-985. 3. Hachimi, M., Grabowski, C., Campanario, S., Herranz, G., Baonza, G., Serrador, J. M., ... & Martin-Belmonte, F. (2021). Smoothelin-like 2 inhibits coronin-1B to stabilize the apical actin cortex during epithelial morphogenesis. Current Biology, 31(4), 696-706.

Uniprot ID: Q2TAL5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 342527

Protein Name: Smoothelin-like protein 2

Gene Full Name: smoothelin like 2

Synonyms: smoothelin like 2; Smoothelin like protein 2; SMTNL2